Cells were treated with 10 ng of the prototype PAMP, lipopolysaccharide (LPS)

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[ Senolytics reduce coronavirus-related mortality in old mice ]

(A) Human adipocyte progenitors isolated from subcutaneous fat biopsies were induced to undergo senescence with 10 Gy of ionizing radiation (SnC) or not (non-SnC) (n = 5 subjects). Cells were treated with 10 ng of the prototype PAMP, lipopolysaccharide (LPS) for 3 hours before RNA isolation. Gene expression was measured by qPCR and the expression in LPS-treated cells normalized to vehicle-treated samples. Means ‘ SEM. Statistical significance was calculated using a mixed effect model for the effect of LPS on SnC and its differential effects on SnC compared to non-SnC. Supplemental table S1 has details. Arrows and asterisks: gray = vehicle-treated SnC vs. non-SnC; black = LPS-treated SnC vs. non-SnC; red = SnC ‘ LPS. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. (B) Young (2-month-old) and old (26-month-old) mice were treated with PBS (n = 5y and 5o) or LPS (n = 4y and 3o) and tissues collected 24 hours later. RNA was isolated from liver and gene expression measured by qPCR. Expression in LPS-treated mice was normalized to vehicle-treated animals. Means ' SEM, two-way ANOVA and post-hoc comparison Tukey's Honestly Significant Difference used to compare the two animal cohorts within a treatment group. Arrows and asterisks: gray = vehicle-treated old vs. young; black = LPS-treated old vs. young; red = old ' LPS. **p < 0.01, ***p < 0.001, ****p < 0.0001. Kidney data in fig. S2. (C) Serum protein from the same mice measured by ELISA. Statistics as described in B.

Publisher: Science
Date: 2021-06-08
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Many things are taking place:

Senolytics reduce COVID-19 symptoms in preclinical studies

‘Even though vaccine use is growing, senolytics could still be helpful to those who cannot receive the vaccine, and especially to older individuals in nursing homes with comorbidities or immunity issues,’ says James Kirkland, M.D., Ph.D., director of the Kogod Center on Aging and, together with Tamar Tchkonia, Ph.D., senior author for Mayo Clinic on the study. The study suggests senolytics could also improve response of the elderly to vaccines and help them fight bacterial and other viral infections.


Publisher: Mirage News
Date: 2021-06-09T00:48:13+10:00
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Vaping Exacerbates Coronavirus-Related Pulmonary Infection in a Murine Model

Unless otherwise noted, all reagents and materials were purchased from either Thermo Fisher Scientific (Waltham, MA, United States) or Sigma-Aldrich (St. Louis, MO, United States) at the highest level of purity possible. A 50-mM 2-APB stock was first made up in DMSO, which was then diluted with sterile PBS to 500 ‘M, as has been previously described for the intravenous use of 2-APB in a mouse model (Morihara et al., 2017).

The e-liquids (‘Mint’ JUUL pods) were purchased locally from retailers in Durham, NC, United States, between July 1, 2020 and October 15, 2020. These products were inventoried and stored at room temperature until used. Manufacturer’s label information stated that ingredients include only vegetable glycerin (VG), propylene glycol (PG), nicotine, flavoring, and benzoic acid, with each pod containing 0.7 ml of the flavored fluid at 3% nicotine.

Publisher: www.frontiersin.org
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Plague of ravenous, destructive mice tormenting Australians

BOGAN GATE, Australia (AP) ‘ At night, the floors of sheds vanish beneath carpets of scampering mice. Ceilings come alive with the sounds of scratching. One family blamed mice chewing electrical wires for their house burning down.

Vast tracts of land in Australia’s New South Wales state are being threatened by a mouse plague that the state government describes as ‘absolutely unprecedented.’ Just how many millions of rodents have infested the agricultural plains across the state is guesswork.

‘We’re at a critical point now where if we don’t significantly reduce the number of mice that are in plague proportions by spring, we are facing an absolute economic and social crisis in rural and regional New South Wales,’ Agriculture Minister Adam Marshall said this month.

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Publisher: KXAN Austin
Date: 2021-05-28T03:37:37+00:00
Author: ROD McGUIRK Associated Press
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Australians ‘fatigued’ from dealing with unprecedented mice plague

Bruce Barnes said he is taking a gamble by planting crops on his family farm near the central New South Wales town of Bogan Gate.

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The risk is that the mice will maintain their numbers through the Southern Hemisphere winter and devour the wheat, barley and canola before it can be harvested.

NSW Farmers, the state’s top agricultural association, predicts the plague will wipe more than 1 billion Australian dollars ($775 million) from the value of the winter crop.

‘We’re having to go down this path because we need something that is super strength, the equivalent of napalm to just blast these mice into oblivion,’ Marshall said.

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Publisher: KTLA
Date: 2021-05-28T11:28:43+00:00
Author: Associated Press
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From Kaiser Health News – Latest Stories:

In the latest sign that in-home acute care is catching on, two big players ‘ Kaiser Permanente and the Mayo Clinic ‘ announced plans to collectively invest $100 million into the company Medically Home to help scale up their programs. (Julie Appleby, 5/24)

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Experts offer advice on how seniors struggling with physical, emotional and cognitive challenges following a year of being cooped up can address issues such as muscle weakness, poor nutrition, disrupted sleep, anxiety and social isolation. (Judith Graham, 5/24)

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No-shows for behavioral health appointments have been a long-standing problem, with up to 60% skipped. Now telehealth, fueled by the pandemic, makes it easier for people dealing with depression and other mental health issues to make it to their appointments at a time when such care is in high demand. But teletherapy creates other challenges. (Eric Berger, 5/24)

Publisher: Kaiser Health News
Date: 2021-05-24T13:10:22+00:00
Twitter: @khnews
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Greetings Earthlings: Those crazy UFOs again! The data presented above may one day be zapped to another dimension. Just thought you should be aware. Hey, buddy, why are all the planets not aligning?